The Alexander Hamilton U.S. Custom House is a building in New York City built 1902–1907 by the federal government to house the duty collection operations for the port of New York. It is located near the southern tip of Manhattan, roughly on the same spot as Fort Amsterdam, the original centre of the settlement of New Amsterdam. Its address is 1 Bowling Green. The building is now the home of the New York branch of the National Museum of the American Indian as well as the Bankruptcy Court for the Southern District of New York. As of 2012, it is also the home to the National Archives at New York City.

The building is on the National Register of Historic Places, and for both exterior and public interior spaces. The Customs House was one of the earliest designations of the New York City Landmarks Preservation Commission, so in 1987 the completion of its preservation, spurred by Senator Daniel Patrick Moynihan who saved the building from demolition in 1979, attracted much public attention: exterior and ceremonial interior spaces were cleaned, restored, and conserved, while old office space was renovated for Federal courtrooms and ancillary offices, for rental offices and meeting rooms, and for a 350-seat auditorium with state-of-the-art projection facilities. Upgrades of fire-safety, security, telecommunications, and heating, air conditioning, and ventilating systems accompanied alterations.

Films that feature the US Custom House include: